How the Oligo Resuspension Calculator Works

The Resuspension Calculator helps you determine the volume of solvent required to resuspend an oligonucleotide (oligo) to a specified concentration. It is particularly useful for preparing DNA or RNA samples for experiments.

Inputs:

Mass of Oligo: Enter the mass of the oligo in units of micrograms (µg), nanograms (ng), or milligrams (mg).

Target Concentration: Specify the desired concentration in micromolar (µM).

Oligo Length: Input the length of the oligonucleotide in bases (e.g., the number of nucleotides).

How to calculate the resuspension volume:

The calculator uses the formula below to estimate the volume needed for resuspension:

Volume (µL) = (Mass × Molecular Weight) / (Concentration × 10⁶)

Where:

Mass: The mass of the oligo, converted into grams.
Molecular Weight: Calculated as 330 g/mol × Length (in bases) (approximation for average nucleotides).
Concentration: Desired target concentration in micromoles per liter (µM).

Why Use Our Resuspension Calculator?

Our Resuspension Calculator offers several benefits:

Accuracy: Precisely calculates solvent volume based on your input values.
Ease of Use: User-friendly interface that requires minimal input.
Instant Results: Get results in real-time with clear output.

Frequently Asked Questions (FAQs)

What is the molecular weight used for calculations?

The molecular weight is estimated based on an average value of 330 g/mol per nucleotide. For a more precise calculation, you can adjust this value to match the specific sequence composition.

What if the calculated volume is very small or impractical?

If the calculated volume is too small (e.g., less than 1 µL), the calculator will display a warning. Consider reducing the target concentration or increasing the mass of the oligo to achieve a more practical volume.

Can I use this calculator for RNA oligos?

Yes, you can use this calculator for RNA oligos. However, RNA oligos may have slightly different molecular weights due to the presence of a hydroxyl group at the 2' position.

What units are supported for input?

The calculator supports mass inputs in nanograms (ng), micrograms (µg), or milligrams (mg) and outputs volume in microliters (µL).